Journal article
Functional expression and pharmacological characterization of the human EAA4 (GluR6) glutamate receptor: A kainate selective channel subunit
KH Hoo, SL Nutt, EJ Fletcher, CE Elliott, B Korczak, RM Deverill, V Rampersad, RP Fantaske, RK Kamboj
Receptors and Channels | HARWOOD ACAD PUBL GMBH | Published : 1994
Abstract
A cDNA encoding an ionotropic glutamate receptor subunit protein humEAA4 (GluR6), has been cloned from a human fetal brain library. This cDNA when expressed in COS or HEK-293 cells is associated with high-affinity kainate receptor binding and ion channel formation. We have successfully established cell lines stably expressing humEAA4 in HEK-293 cells This is the first report of the establishment of stable cell lines expressing a glutamate receptor channel. The relative potency of compounds for displacing [3H]-kainate binding to humEAA4 receptors expressed in COS or HEK-293 cells is domoate > kainate > quisqualate > 6-cyano-7-nitroquinoxaline-2,3-dione > L-glutamate = 6,7- dinitroquinoxaline-..
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