Journal article

The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation

Hua-Chen Chang, Sarita Sehra, Ritobrata Goswami, Weiguo Yao, Qing Yu, Gretta L Stritesky, Rukhsana Jabeen, Carl McKinley, Ayele-Nati Ahyi, Ling Han, Evelyn T Nguyen, Michael J Robertson, Narayanan B Perumal, Robert S Tepper, Stephen L Nutt, Mark H Kaplan

NATURE IMMUNOLOGY | NATURE PUBLISHING GROUP | Published : 2010

Abstract

CD4(+) helper T cells acquire effector phenotypes that promote specialized inflammatory responses. We show that the ETS-family transcription factor PU.1 was required for the development of an interleukin 9 (IL-9)-secreting subset of helper T cells. Decreasing PU.1 expression either by conditional deletion in mouse T cells or the use of small interfering RNA in human T cells impaired IL-9 production, whereas ectopic PU.1 expression promoted IL-9 production. Mice with PU.1-deficient T cells developed normal T helper type 2 (T(H)2) responses in vivo but showed attenuated allergic pulmonary inflammation that corresponded to lower expression of Il9 and chemokines in peripheral T cells and in lung..

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University of Melbourne Researchers

Grants

Awarded by National Institutes of Health


Awarded by NATIONAL CANCER INSTITUTE


Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

Supported by the National Institutes of Health (R01 AI57459 and U19 AI070448 to M. H. K., R01 CA118118 to M.J.R.; R01 HL080071 to R. S. T.; and T32 AI060519 to G. L. S.).