Mechanisms of Palmitate-Induced Lipotoxicity in Human Osteoblasts
Krishanthi Gunaratnam, Christopher Vidal, Jeffrey M Gimble, Gustavo Duque
ENDOCRINOLOGY | ENDOCRINE SOC | Published : 2014
The interest in the relationship between fat and bone has increased steadily during recent years. Fat could have a lipotoxic effect on bone cells through the secretion of fatty acids. Palmitate is the most prevalent fatty acid secreted by adipocytes in vitro. Considering that palmitate has shown a high lipotoxic effect in other tissues, here we characterized the lipotoxic effect of palmitate on human osteoblasts (Obs). Initially we tested for changes in palmitoylation in this model. Subsequently we compared the capacity of Obs to differentiate and form bone nodules in the presence of palmitate. From a mechanistic approach, we assessed changes in nuclear activity of β-catenin and runt-related..View full abstract
Awarded by Australian National Health and Medical Research Council
This work was supported by the Australian National Health and Medical Research Council (Grant 632767) and the Nepean Medical Research Foundation.