Validation of noninvasive quantification of bone marrow fat volume with microCT in aging rats
Oddom Demontiero, Wei Li, Emma Thembani, Gustavo Duque
Experimental Gerontology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2011
Marrow fat infiltration is one of the hallmarks of age-related bone loss. This fat infiltration has been quantified by invasive and noninvasive methods. However, the validity of the noninvasive methods has not been correlated with a gold standard. In this study we aim to validate the usefulness of marrow fat quantification by correlating microCT (μCT) images with histology analysis. Fat volume (FV) and bone volume (BV) of distal femora of young (4 months) and old (27 months) Louvain/c (LOU) rats (n=22) were quantified by histology and compared with μCT images analyzed by an image analysis software (SliceOMatic). We found that for SliceOMatic/μCT the intra-rater reliability for duplicate meas..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
This study was supported by a project grant from the Australian National Health and Medical Research Council (NHMRC, Grant # 632767) and by an operating grant from the Nepean Medical Research Foundation. A/Prof. Duque and Dr Li hold Fellowships from the University of Sydney Medical Research Foundation. Dr Demontiero holds a scholarship from The Rebecca Cooper Medical Research Foundation. The authors are deeply grateful to the members of the Quebec Network for Research in Aging for providing us with samples from their LOU rat colony.