Journal article

Nucleosome levels and toll-like receptor expression during high cut-off haemofiltration: a pilot assessment

Rafidah Atan, Clive May, Simon R Bailey, Marcel Tanudji, Kumar Visvanathan, Narelle Skinner, Rinaldo Bellomo, Hermann Goehl, Markus Storr

CRITICAL CARE AND RESUSCITATION | AUSTRALASIAN MED PUBL CO LTD | Published : 2015

Abstract

OBJECTIVES: To measure plasma nucleosome levels and expression of toll-like receptors (TLRs) in a pilot cohort of patients with severe acute kidney injury (AKI) within a randomised controlled trial of continuous venovenous haemofiltration with high cut-off filters (CVVH-HCO) v standard filters (CVVH-std). METHODS: We measured plasma nucleosome levels using the Cell Death Detection ELISA PLUS (10X) assay kit. We analysed plasma levels for correlation with disease severity and compared the effects of CVVH-HCO and CVVH-std on plasma nucleosome levels over the first 72 hours. We studied cell surface TLR expression on CD14-positive monocytes in a subcohort of CVVH-HCO patients. RESULTS: We did no..

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