Site and mechanism of the colokinetic action of the ghrelin receptor agonist, HM01
K Naitou, TP Mamerto, RV Pustovit, B Callaghan, LR Rivera, AJ Chan, MT Ringuet, C Pietra, JB Furness
Neurogastroenterology & Motility | WILEY | Published : 2015
BACKGROUND: It has been recently demonstrated that the ghrelin receptor agonist, HM01, caused defecation in rats that were treated to provide a model for the constipation of Parkinson's disease. HM01 significantly increased fecal output and increased Fos activity in neurons of the hypothalamus and hindbrain, but not in the spinal defecation center. Other ghrelin agonists act on the defecation center. METHODS: Receptor pharmacology was examined in ghrelin receptor (GHSR1a) transfected cells. Anesthetized rats were used to investigate sites and mechanisms of action. KEY RESULTS: HM01 activated rat GHSR1a at nanomolar concentrations and was antagonized by the GHSR1a antagonist, YIL781. HM01, in..View full abstract
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Awarded by National Health and Medical Research Council of Australia
The work was supported by the Transport Accident Commission of Victoria through the Institute for Safety, Compensation and Recovery Research, and by a grant from the National Health and Medical Research Council of Australia (grant number 1005811). KN was supported by the Japan Public-Private Partnership Student Study Abroad Program (TOBITATE! Young Ambassador Program) from the Japan Student Services Organization (JASSO). LRR was supported by an NHMRC early career fellowship.