Journal article
A chemical screening approach to identify novel key mediators of erythroid enucleation
CB Wölwer, LB Pase, HB Pearson, NJ Gödde, K Lackovic, DCS Huang, SM Russell, PO Humbert
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2015
Open access
Abstract
Erythroid enucleation is critical for terminal differentiation of red blood cells, and involves extrusion of the nucleus by orthochromatic erythroblasts to produce reticulocytes. Due to the difficulty of synchronizing erythroblasts, the molecular mechanisms underlying the enucleation process remain poorly understood. To elucidate the cellular program governing enucleation, we utilized a novel chemical screening approach whereby orthochromatic cells primed for enucleation were enriched ex vivo and subjected to a functional drug screen using a 324 compound library consisting of structurally diverse, medicinally active and cell permeable drugs. Using this approach, we have confirmed the role of..
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Awarded by National Health and Medical Research Council (NH&MRC) of Australia
Awarded by Leukemia Lymphoma Society
Funding Acknowledgements
CBW was supported by the Australian Postgraduate Award. DCSH is supported by grants (program 1016701 and Independent Research Institutes Infrastructure Support Scheme grant 361646) and a fellowship (1043149) from the National Health and Medical Research Council (NH&MRC) of Australia; the Leukemia Lymphoma Society (SCoR 7001-13); and a Victorian State Government Operational Infrastructure Support (OIS) grant. SMR was supported by grants and fellowships from the National Health and Medical Research Council and Australian Research Council (ARC) of Australia. POH was supported by a Senior Research Fellowship and a project grant from the National Health and Medical Research Council of Australia.