Respiratory DC Use IFITM3 to Avoid Direct Viral Infection and Safeguard Virus-Specific CD8( ) T Cell Priming

Giuseppe Infusini; Jeffrey M Smith; He Yuan; Angela Pizzolla; Wy Ching Ng; Sarah L Londrigan; Ashraful Haque; Patrick C Reading; Jose A Villadangos; Linda M Wakim

Journal article

Respiratory DC Use IFITM3 to Avoid Direct Viral Infection and Safeguard Virus-Specific CD8( ) T Cell Priming

Giuseppe Infusini, Jeffrey M Smith, He Yuan, Angela Pizzolla, Wy Ching Ng, Sarah L Londrigan, Ashraful Haque, Patrick C Reading, Jose A Villadangos, Linda M Wakim

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2015

DOI: 10.1371/journal.pone.0143539

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Abstract

Respiratory dendritic cells (DC) play a pivotal role in the initiation of adaptive immune responses to influenza virus. To do this, respiratory DCs must ferry viral antigen from the lung to the draining lymph node without becoming infected and perishing en route. We show that respiratory DCs up-regulate the expression of the antiviral molecule, interferon-induced transmembrane protein 3 (IFITM3) in response to influenza virus infection, in a manner dependent on type I interferon signaling and the transcription factors IRF7 and IRF3. Failure of respiratory DCs to up-regulate IFITM3 following influenza virus infection resulted in impaired trafficking to the draining LN and consequently in impa..

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University of Melbourne Researchers

Angela Pizzolla Author The Sir Peter Maccallum Department of Oncology

Linda Wakim Author Microbiology and Immunology

Patrick Reading Author Microbiology and Immunology

Jose Villadangos Author Biochemistry and Pharmacology

Giuseppe Infusini Author Medical Biology (w.e.h.i.)

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Grants

Funding Acknowledgements

This work was funded by the National Health and Medical Research Council (NHMRC) of Australia, with additional support from the University of Melbourne (Early Career Establishment Grant) and Bio21 Institute/CSL Ltd. J. A. Villadangos is a NHMRC Principal Research Fellow and L. Wakim is a NHMRC Career Development Fellow. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.