An optimised perfusion technique for extracting murine gastric leukocytes

Abstract

The stomach is a difficult tissue to analyse by flow cytometry, largely due to the difficulty of isolating viable leukocytes. Here we present the re-optimization of a perfusion technique that compares favourably against two other methods of enzymatic digestion for the release of gastric leukocytes. We believe that this technique could greatly assist the analysis of immune cells basally present in the murine stomach and that infiltrate during infection or disease.