Journal article

Tailoring minimalist self-assembling peptides for localized viral vector gene delivery

Alexandra L Rodriguez, Ting-Yi Wang, Kiara F Bruggeman, Rui Li, Richard J Williams, Clare L Parish, David R Nisbet



Viral vector gene delivery is a promising technique for the therapeutic administration of proteins to damaged tissue for the improvement of regeneration outcomes in various disease settings including brain and spinal cord injury, as well as autoimmune diseases. Though promising results have been demonstrated, limitations of viral vectors, including spread of the virus to distant sites, neutralization by the host immune system, and low transduction efficiencies have stimulated the investigation of biomaterials as gene delivery vehicles for improved protein expression at an injury site. Here, we show how Nfluorenylmethyloxycarbonyl (Fmoc) self-assembling peptide (SAP) hydrogels, designed for t..

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Awarded by National Health and Medical Research Council, Australia (NHMRC)

Awarded by Australian Research Council (ARC)

Funding Acknowledgements

Access to the facilities of the Centre for Advanced Microscopy (CAM) with funding through the Australian Microscopy and Microanalysis Research Facility (AMMRF) is gratefully acknowledged. We acknowledge NanoScope-Scientific Graphics and Illustration for kindly designing the virus immobilization schematic in Fig. 3. We would also like to thank Conor Horgan, Francesca Maclean and Anitha Parnneerselvan for thorough proof reading. Funding for this research was obtained from the National Health and Medical Research Council, Australia (NHMRC, No. APP1050684), and the Australian Research Council (ARC, No. DP130103131). A. L. R. was supported by an Australian Postgraduate Award; R. J. W. was funded via an Alfred Deakin Research Fellowship; C. L. P. was supported by Senior Medical Research Fellowship provided by the Viertel charitable Foundation, Australia; D. R. N. was supported by an ARC Australian Postdoctoral Fellowship, followed by an NHMRC Career Development Fellowship. The Florey Institute of Neuroscience and Mental Health acknowledges the support from the Victorian Government's Operational Infrastructure Support Grant.