Journal article

Localization of dipeptidyl peptidase-4 (CD26) to human pancreatic ducts and islet alpha cells

Petra Augstein, Gaetano Naselli, Thomas Loudovaris, Wayne J Hawthorne, Peter Campbell, Esther Bandala-Sanchez, Kelly Rogers, Peter Heinke, Helen E Thomas, Thomas W Kay, Leonard C Harrison

Diabetes Research and Clinical Practice | ELSEVIER IRELAND LTD | Published : 2015

Abstract

AIM: DPP-4/CD26 degrades the incretins GLP-1 and GIP. The localization of DPP-4 within the human pancreas is not well documented but is likely to be relevant for understanding incretin function. We aimed to define the cellular localization of DPP-4 in the human pancreas from cadaveric organ donors with and without diabetes. METHODS: Pancreas was snap-frozen and immunoreactive DPP-4 detected in cryosections using the APAAP technique. For co-localization studies, pancreas sections were double-stained for DPP-4 and proinsulin or glucagon and scanned by confocal microscopy. Pancreata were digested and cells in islets and in islet-depleted, duct-enriched digests analyzed for expression of DPP-4 a..

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Grants

Awarded by NHMRC-JDRF


Awarded by NHMRC


Funding Acknowledgements

P.A. was the recipient of EFSD Albert Renold Travel Fellowship from the European Federation for the Study of Diabetes. This research was supported by a NHMRC-JDRF Special Program grant (465199). The authors are grateful to Mrs. Alana Neale for technical assistance and to Dr. Matthias Hundt for advice about image analysis. The work was made possible through Victorian State Government Operational Infrastructure Support and Australian National Health and Medical Research Council Research Institute Infrastructure Support Scheme. L.C.H. was the recipient of a Senior Principal Research Fellowship (637301) of the NHMRC.