Journal article
Successful treatment of biofilm infections using shock waves combined with antibiotic therapy
DP Gnanadhas, M Elango, S Janardhanraj, CS Srinandan, A Datey, RA Strugnell, J Gopalan, D Chakravortty
Scientific Reports | Published : 2015
DOI: 10.1038/srep17440
Abstract
Many bacteria secrete a highly hydrated framework of extracellular polymer matrix on suitable substrates and embed within the matrix to form a biofilm. Bacterial biofilms are observed on many medical devices, endocarditis, periodontitis and lung infections in cystic fibrosis patients. Bacteria in biofilm are protected from antibiotics and >1,000 times of the minimum inhibitory concentration may be required to treat biofilm infections. Here, we demonstrated that shock waves could be used to remove Salmonella, Pseudomonas and Staphylococcus biofilms in urinary catheters. The studies were extended to a Pseudomonas chronic pneumonia lung infection and Staphylococcus skin suture infection model i..
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Awarded by Department of Science and Technology, Ministry of Science and Technology, India
Funding Acknowledgements
We thank the Electron microscopy facility, IISc for the help. We thank the Central Animal Facility, IISc for providing the mice. This work was supported by the grant Provision (2A) Tenth Plan (191/MCB) from the director of the Indian Institute of Science, Bangalore, India, and the Department of Biotechnology (DBT 311, NBA, sanctioned by the President), Life Science Research Board (LSRB0008) and DBT-IISc partnership program for advanced research in biological sciences and bioengineering to DC Infrastructure support from ICMR (Center for Advanced Study in Molecular Medicine), DST (FIST), and UGC (special assistance) is acknowledged. DPG, AD and JS are research scholars and ME is project assistant. CSS is a DBT RA post-doc fellow. RAS is supported by the AD acknowledges DBT for Senior Research Fellowship.