Journal article

Practical and low cost whole-organism motility assay: A step-by-step protocol

Sarah Preston, Abdul Jabbar, Cameron Nowell, Anja Joachim, Baerbel Ruttkowski, Tony Cardno, Andreas Hofmann, Robin B Gasser

MOLECULAR AND CELLULAR PROBES | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2016

Abstract

Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds for activity against parasitic worms. This assay has considerable advantages over conventional methods, mainly in relation to ease of use, throughput, time and cost. It is readily suited to the screening of hundreds to thousands of compounds for subsequent hit-to-lead optimisation, and should be applicable to many different parasites and other organisms commensurate with the size of wells in the microtiter plates used for phenotypic screening.

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Grants

Awarded by Victorian Life Sciences Computation Initiative (VLSCI) Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government


Funding Acknowledgements

This work was supported by the Australian Research Council, the National Health and Medical Research Council of Australia and the Victorian Life Sciences Computation Initiative (VLSCI) grant number VR0007 on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government.