Journal article
The wiggle index: An open source bioassay to assess sub-lethal insecticide response in Drosophila melanogaster
S Denecke, CJ Nowell, A Fournier-Level, T Perry, P Batterham
Plos One | Published : 2015
Abstract
Toxicological assays measuring mortality are routinely used to describe insecticide response, but sub-lethal exposures to insecticides can select for resistance and yield additional biological information describing the ways in which an insecticide impacts the insect. Here we present the Wiggle Index (WI), a high-throughput method to quantify insecticide response by measuring the reduction in motility during sub-lethal exposures in larvae of the vinegar fly Drosophila melanogaster. A susceptible wild type strain was exposed to the insecticides chlorantraniliprole, imidacloprid, spinosad, and ivermectin. Each insecticide reduced larval motility, but response times and profiles differed among ..
View full abstractGrants
Awarded by Australian Research Council
Funding Acknowledgements
This research was supported by the Australian Research Council (Discovery Project Grant DP130102414), http://www.arc.gov.au/. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.r The authors would like to thank the Basler lab, the Bloomington stock centre and the Drosophila Genome Resource Center for the provision of D. melanogaster stocks and the Australian Drosophila Biomedical Research Support Facility for handling the quarantine of imported flies. This research was supported by funding from the Australian Research Council (Discovery Project Grant DP130102414) awarded to PB.