Time-Dependent Transcriptional Changes in Axenic Giardia duodenalis Trophozoites
Brendan RE Ansell, Malcolm J McConville, Louise Baker, Pasi K Korhonen, Neil D Young, Ross S Hall, Cristian AA Rojas, Staffan G Svard, Robin B Gasser, Aaron R Jex
PLoS Neglected Tropical Diseases | PUBLIC LIBRARY SCIENCE | Published : 2015
Giardia duodenalis is the most common gastrointestinal protozoan parasite of humans and a significant contributor to the global burden of both diarrheal disease and post-infectious chronic disorders. Although G. duodenalis can be cultured axenically, significant gaps exist in our understanding of the molecular biology and metabolism of this pathogen. The present study employed RNA sequencing to characterize the mRNA transcriptome of G. duodenalis trophozoites in axenic culture, at log (48 h of growth), stationary (60 h), and declining (96 h) growth phases. Using ~400-times coverage of the transcriptome, we identified 754 differentially transcribed genes (DTGs), mainly representing two large ..View full abstract
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Awarded by Australian Research Council
BREA is supported by an Australian Post-graduate Award (Australian Government) and the Victorian Life Sciences Computation Initiative (Victoria, Australia). MJM and ARJ are partially supported by an Australian Research Council Linkage Grant (number LP120200122). RNA sequencing was partially funded by YourGene Biosciences (Taiwan). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.