Increased detection in Australia and Singapore of a novel influenza A(H1N1) 2009 variant with reduced oseltamivir and zanamivir sensitivity due to a S247N neuraminidase mutation
AC Hurt, RT Lee, SK Leang, L Cui, YM Deng, SP Phuah, N Caldwell, K Freeman, N Komadina, D Smith, D Speers, A Kelso, RT Lin, S Maurer-Stroh, IG Barr
EUROSURVEILLANCE | EUR CENTRE DIS PREVENTION & CONTROL | Published : 2011
A novel influenza A(H1N1)2009 variant with mildly reduced oseltamivir and zanamivir sensitivity has been detected in more than 10% of community specimens in Singapore and more than 30% of samples from northern Australia during the early months of 2011. The variant, which has also been detected in other regions of the Asia-Pacific, contains a S247N neuraminidase mutation. When combined with the H275Y mutation, as detected in an oseltamivir-treated patient, the dual S247N+H275Y mutant had extremely high oseltamivir resistance.
The authors wish to thank the various laboratories that submit specimens and isolates to the Melbourne WHO Collaborating Centre for Reference and Research on Influenza. The reverse genetics plasmid pHW2000 was kindly provided by Dr Richard Webby, St Jude Children's Research Hospital, Memphis, United States. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health and Ageing.