T Cell Help Amplifies Innate Signals in CD8( ) DCs for Optimal CD8( ) T Cell Priming
Marie Greyer, Paul G Whitney, Angus T Stock, Gayle M Davey, Christina Tebartz, Annabell Bachem, Justine D Mintern, Richard A Strugnell, Stephen J Turner, Thomas Gebhardt, Meredith O'Keeffe, William R Heath, Sammy Bedoui
CELL REPORTS | CELL PRESS | Published : 2016
DCs often require stimulation from CD4(+) T cells to propagate CD8(+) T cell responses, but precisely how T cell help optimizes the priming capacity of DCs and why this appears to differ between varying types of CD8(+) T cell immunity remains unclear. We show that CD8(+) T cell priming upon HSV-1 skin infection depended on DCs receiving stimulation from both IFN-α/β and CD4(+) T cells to provide IL-15. This was not an additive effect but resulted from CD4(+) T cells amplifying DC production of IL-15 in response to IFN-α/β. We also observed that increased innate stimulation reversed the helper dependence of CD8(+) T cell priming and that the innate stimulus, rather than the CD4(+) T cells the..View full abstract
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Awarded by National Health and Medical Research Council of Australia
Awarded by German Research Council
We would like to thank Drs. P.J. Hertzog, A.M. Lew, and R.A. Flavell for reagents, mice, and helpful discussions. The excellent assistance by Amanda Turner is gratefully acknowledged. Our research is supported by the National Health and Medical Research Council of Australia (APP1013641 to P.G.W.), the Australian Research Council, and the German Research Council (BA5108/1-1 to A.B.).