Salmonella typhimurium ΔaroA ΔaroD mutants expressing a foreign recombinant protein induce specific major histocompatibility complex class I- restricted cytotoxic T lymphocytes in mice

Abstract

Recombinant Salmonella typhimurium aroA aroD mutants which expressed ovalbumin were constructed. The two expression constructs used were based on either pUC18 or pBR322. The pBR322-based construct was more stable in vitro and in vivo than the pUC-based construct. Salmonellae containing the stable pBR322-based plasmid induced major histocompatibility complex (MHC) class-I- restricted cytotoxic T lymphocytes (CTL), in contrast to salmonellae containing the pUC18-based expression construct. The priming of MHC class I- restricted CTL was increased by multiple immunizations. The study described in this report suggests that S. typhimurium Δaro mutants have the capacity to induce MHC class I-restri..