1‐O‐hexadecyl‐2‐acetyl‐sn‐glycero‐3‐phospho (N,N,N trimethyl) hexanolamine: an analogue of platelet‐activating factor with partial agonist activity

Abstract

During studies of the role of platelet‐activating factor (PAF) in macrophage superoxide anion generation (O2−1), we identified an agonist action of the putative PAF receptor antagonist 1‐O‐hexadecyl‐2‐acetyl‐sn‐glycero‐3‐phospho (N,N,N‐trimethyl) hexanolamine (hexanolamine PAF) in guinea‐pig macrophages. The 1‐O‐octadecyl form of this compound has specific antagonist actions at PAF receptors. The agonist properties of hexanolamine PAF were examined in rabbit washed platelets (aggregation) and in guinea‐pig peritoneal macrophages (O2−generation). Hexanolamine PAF induced significant platelet aggregation (50% of the PAF maximum). However, the omission of bovine serum albumin (BSA) from the Tyr..