Journal article

ENDOTHELIUM-DERIVED RELAXING FACTOR RELEASED FROM CULTURED-CELLS - DIFFERENTIATION FROM NITRIC-OXIDE

GJ DUSTING, MA READ, AG STEWART

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY | BLACKWELL SCIENCE | Published : 1988

Abstract

1. Endothelium-derived relaxing factor (EDRF) is an extremely labile mediator thought to be identical to nitric oxide (NO). 2. A cascade superfusion technique was used to bioassay EDRF released from bovine aortic endothelial cells grown to confluence on microcarrier beads. 3. Bradykinin (1-100 nmol/l), infused through a 1 cm column of endothelial cells on microcarriers, released an EDRF-like substance that caused relaxations of de-endothelialized strips of rabbit aorta (precontracted with phenylephrine). These relaxations diminished on successive tissues in the cascade, when compared with those produced by glyceryl trinitrate as a stable standard. 4. Haemoglobin (1 mumol/l), infused directly..

View full abstract