Analysis of microRNA turnover in mammalian cells following Dicer1 ablation
Michael P Gantier, Claire E McCoy, Irina Rusinova, Damien Saulep, Die Wang, Dakang Xu, Aaron T Irving, Mark A Behlke, Paul J Hertzog, Fabienne Mackay, Bryan RG Williams
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2011
Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryonic fibroblasts following loss of Dicer1 enzymatic activity. The results confirm the inherent stability of miRNAs, the intracellular levels of which were mostly affected by cell division. Using the decay rates of a panel of six miRNAs representative of the global trend of miRNA decay, we establish a mathematical model of miRNA turnover and determine an average miRNA half-life of 119 h (i.e. ∼5 day..View full abstract
Awarded by The Australian National Health and Medical Research Council
The Australian National Health and Medical Research Council (491106, 606425 and 1006590 to B. R. G. W.); Victorian Government's Operational Infrastructure Support Program; Health Research Board Ireland (to C. E. M.). Funding for open access charge: Monash Institute of Medical Research.