Journal article
FOXO1/3 and PTEN depletion in granulosa cells promotes ovarian granulosa cell tumor development
Z Liu, YA Ren, SA Pangas, J Adams, W Zhou, DH Castrillon, D Wilhelm, JS Richards
Molecular Endocrinology | Published : 2015
DOI: 10.1210/me.2015-1103
Abstract
The forkhead box (FOX), FOXO1 and FOXO3, transcription factors regulate multiple functions in mammalian cells. Selective inactivation of the Foxo1 and Foxo3 genes in murine ovarian granulose cells severely impairs follicular development and apoptosis causing infertility, and as shown here, granulosa cell tumor (GCT) formation. Coordinate depletion of the tumor suppressor Pten gene in the Foxo1/3 strain enhanced the penetrance and onset of GCT formation. Immunostaining and Western blot analyses confirmed FOXO1 and phosphatase and tensin homolog (PTEN) depletion, maintenance of globin transcription factor (GATA) 4 and nuclear localization of FOXL2 and phosphorylated small mothers against decap..
View full abstractGrants
Awarded by National Institute of Child Health and Human Development
Funding Acknowledgements
Ligand Assay and Analysis Core is supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development/National Institutes of Health through cooperative agreement (U54-HD28934) as part of the Specialized Cooperative Centers Program in Reproduction and Infertility Research.