Calcium Signaling Is Required for Erythroid Enucleation
Christina B Woelwer, Luke B Pase, Sarah M Russell, Patrick O Humbert
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2016
Although erythroid enucleation, the property of erythroblasts to expel their nucleus, has been known for 7ore than a century, surprisingly little is known regarding the molecular mechanisms governing this unique developmental process. Here we show that similar to cytokinesis, nuclear extrusion requires intracellular calcium signaling and signal transduction through the calmodulin (CaM) pathway. However, in contrast to cytokinesis we found that orthochromatic erythroblasts require uptake of extracellular calcium to enucleate. Together these functional studies highlight a critical role for calcium signaling in the regulation of erythroid enucleation.
C.B.W. was supported by the Australian Postgraduate Award. P.O.H. and S.M.R. are supported by a Senior Research Fellowship from the NH&MRC and the work by a project grant from the NH&MRC of Australia.