Journal article
G-CSF and Neutrophils Are Nonredundant Mediators of Murine Experimental Autoimmune Uveoretinitis
GL Goldberg, AL Cornish, J Murphy, ES Pang, LL Lim, IK Campbell, K Scalzo-Inguanti, X Chen, PG McMenamin, E Maraskovsky, BS McKenzie, IP Wicks
American Journal of Pathology | Published : 2016
Abstract
Granulocyte colony-stimulating factor (G-CSF) is a regulator of neutrophil production, function, and survival. Herein, we investigated the role of G-CSF in a murine model of human uveitis-experimental autoimmune uveoretinitis. Experimental autoimmune uveoretinitis was dramatically reduced in G-CSF-deficient mice and in anti-G-CSF monoclonal antibody-treated, wild-type (WT) mice. Flow cytometric analysis of the ocular infiltrate in WT mice with experimental autoimmune uveoretinitis showed a mixed population, comprising neutrophils, macrophages, and T cells. The eyes of G-CSF-deficient and anti-G-CSF monoclonal antibody-treated WT mice had minimal neutrophil infiltrate, but no change in other ..
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Funding Acknowledgements
Supported by The Ophthalmic Research Institute of Australia, CSL Limited and Reid Charitable Trusts, The National Health and Medical Research Council (Canberra, Australia), Career Development Fellowship grant 1034598 (G.L.G.), Peter Doherty Post-Doctoral Fellowship grant 310608 (A.L.C.), Industry Research Fellowship grant 461287 (I.K.C.), Clinical Practitioner Fellowship grant 1023407 (I.P.W.), program grant 1016647 (I.P.W.), and operational infrastructure grants through the Australian Government Institute for Research and Innovation in Social Services and the Victorian State Government Offer Information Statement.