Journal article

Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells

Damian Hernandez, Rodney Millard, Priyadharshini Sivakumaran, Raymond CB Wong, Duncan E Crombie, Alex W Hewitt, Helena Liang, Sandy SC Hung, Alice Pebay, Robert K Shepherd, Gregory J Dusting, Shiang Y Lim



Background. Human induced pluripotent stem cells (iPSCs) are an attractive source of cardiomyocytes for cardiac repair and regeneration. In this study, we aim to determine whether acute electrical stimulation of human iPSCs can promote their differentiation to cardiomyocytes. Methods. Human iPSCs were differentiated to cardiac cells by forming embryoid bodies (EBs) for 5 days. EBs were then subjected to brief electrical stimulation and plated down for 14 days. Results. In iPS(Foreskin)-2 cell line, brief electrical stimulation at 65 mV/mm or 200 mV/mm for 5 min significantly increased the percentage of beating EBs present by day 14 after plating. Acute electrical stimulation also significant..

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Awarded by National Health and Medical Research Council of Australia

Funding Acknowledgements

These studies were supported by grants from the National Heart Foundation and National Health and Medical Research Council of Australia (NHMRC 1024817). Duncan E. Crombie is supported by an NHMRC Gustav Nossal Scholarship, Gregory J. Dusting is a Principal Research Fellow of the NHMRC, and Alice Pebay is supported by a NHMRC Career Development Award Fellow and an Australian Research Council Future Fellowship. Raymond C. B. Wong is supported by the Cranbourne Foundation Fellowship and NHMRC Project grant. Support was also provided by the J.R. and J.O. Wicking Trust and Friedreich's Ataxia Research Alliance (research grant and 2012 Keith Michael Andrus Cardiac Research Award). The O'Brien Institute, the Centre for Eye Research Australia, and the Bionics Institute receive Operational Infrastructure Support from the Victorian State Government's Department of Innovation, Industry and Regional Development. The authors thank James A. Thomson (University of Wisconsin) for providing the iPS(Foreskin) cell line and Owen Burns (Bionic Institute) for manufacturing the well-based electrode arrays.