Journal article

Endocytic function is critical for influenza A virus infection via DC-SIGN and L-SIGN

Leah Gillespie, Paula Roosendahl, Wy Ching Ng, Andrew G Brooks, Patrick C Reading, Sarah L Londrigan

SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2016

Abstract

The ubiquitous presence of cell-surface sialic acid (SIA) has complicated efforts to identify specific transmembrane glycoproteins that function as bone fide entry receptors for influenza A virus (IAV) infection. The C-type lectin receptors (CLRs) DC-SIGN (CD209) and L-SIGN (CD209L) enhance IAV infection however it is not known if they act as attachment factors, passing virions to other unknown receptors for virus entry, or as authentic entry receptors for CLR-mediated virus uptake and infection. Sialic acid-deficient Lec2 Chinese Hamster Ovary (CHO) cell lines were resistant to IAV infection whereas expression of DC-SIGN/L-SIGN restored susceptibility of Lec2 cells to pH- and dynamin-depend..

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Grants

Awarded by National Health and Medical Research Council (NHMRC) of Australia


Funding Acknowledgements

This study was supported by project grant 1027545 from the National Health and Medical Research Council (NHMRC) of Australia. P.C.R., S.L.L. and A.G.B are all recipients of funding from the NHMRC. W.N. is a recipient of a NHMRC Dora Lush Biomedical Research Scholarship. S.L.L. is a recipient of a University of Melbourne Early Career Research Grant and a University of Melbourne, Melbourne Research Fellowship. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health.