Journal article
Multifunctional role of the transcription factor Blimp-1 in coordinating plasma cell differentiation
M Minnich, H Tagoh, P Bönelt, E Axelsson, M Fischer, B Cebolla, A Tarakhovsky, SL Nutt, M Jaritz, M Busslinger
Nature Immunology | Published : 2016
DOI: 10.1038/ni.3349
Abstract
The transcription factor Blimp-1 is necessary for the generation of plasma cells. Here we studied its functions in plasmablast differentiation by identifying regulated Blimp-1 target genes. Blimp-1 promoted the migration and adhesion of plasmablasts. It directly repressed genes encoding several transcription factors and Aicda (which encodes the cytidine deaminase AID) and thus silenced B cell-specific gene expression, antigen presentation and class-switch recombination in plasmablasts. It directly activated genes, which led to increased expression of the plasma cell regulator IRF4 and proteins involved in immunoglobulin secretion. Blimp-1 induced the transcription of immunoglobulin genes by ..
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Awarded by Boehringer Ingelheim
Funding Acknowledgements
We thank D. Kostanova Poliakova for technical assistance; K. Aumayr and colleagues for sorting by flow cytometry; K. Mechtler and colleagues for mass spectrometry; A. Sommer and colleagues for Illumina sequencing at the Vienna Biocenter Core Facility; H. Ekker for bioinformatics processing of ATAC-seq data; R. Kingston (Massachusetts General Hospital) for antibody to Brgl; and T. Jenuwein (Max Planck Institute of Immunobiology and Epigenetics, Freiburg) for antibody to H3K27me3. Supported by Boehringer Ingelheim, the European Research Council (European Union Seventh Framework Program: 291740-LymphoControl), the Austrian Industrial Research Promotion Agency, the Emerald Foundation (Tarakhovsky laboratory), the National Health and Medical Research Council of Australia (361646 and 1054925 for the Nutt laboratory) and the Multiple Myeloma Research Foundation (for the Nutt laboratory).