Journal article
Capture and On-chip analysis of Melanoma Cells Using Tunable Surface Shear forces
SCH Tsao, R Vaidyanathan, S Dey, LG Carrascosa, C Christophi, J Cebon, MJA Shiddiky, A Behren, M Trau
Scientific Reports | NATURE PORTFOLIO | Published : 2016
DOI: 10.1038/srep19709
Abstract
With new systemic therapies becoming available for metastatic melanoma such as BRAF and PD-1 inhibitors, there is an increasing demand for methods to assist with treatment selection and response monitoring. Quantification and characterisation of circulating melanoma cells (CMCs) has been regarded as an excellent non-invasive candidate but a sensitive and efficient tool to do these is lacking. Herein we demonstrate a microfluidic approach for melanoma cell capture and subsequent on-chip evaluation of BRAF mutation status. Our approach utilizes a recently discovered alternating current electrohydrodynamic (AC-EHD)-induced surface shear forces, referred to as nanoshearing. A key feature of nano..
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Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by the NHMRC CDF (APP1088966) and ARC DECRA (DE120102503). Although not directly involved in this work, our lab is receiving support from the National Breast Cancer Foundation of Australia (CG-12-07). This grant has significantly contributed to the environment to stimulate the research described here. The fabrication work was performed at Queensland and ACT nodes of the Australian National Fabrication Facility. We would also like to acknowledge the Victorian State Government Operational Infrastructure Support Program for partial funding of the parts of the project based at the Olivia Newton-John Cancer Research Institute. S.C.T is funded by the Australia Postgraduate Award and the Australasian College of Surgeons Foundation for Surgery ANZ journal of Surgery research scholarship.