Journal article
Genetic characterisation of Taenia multiceps cysts from ruminants in Greece
S Al-Riyami, E Ioannidou, AV Koehler, MH Hussain, AH Al-Rawahi, ND Giadinis, SQ Lafi, E Papadopoulos, A Jabbar
Infection Genetics and Evolution | Published : 2016
Abstract
This study was designed to genetically characterise the larval stage (coenurus) of Taenia multiceps from ruminants in Greece, utilising DNA regions within the cytochrome c oxidase subunit 1 (partial cox1) and NADH dehydrogenase 1 (p. nad1) mitochondrial (mt) genes, respectively. A molecular-phylogenetic approach was used to analyse the p. cox1 and p. nad1 amplicons derived from genomic DNA samples from individual cysts (n= 105) from cattle (n= 3), goats (n= 5) and sheep (n= 97). Results revealed five and six distinct electrophoretic profiles for p. cox1 and p. nad1, respectively, using single-strand conformation polymorphism. Direct sequencing of selected amplicons representing each of these..
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Funding Acknowledgements
The Early Career Researcher and Collaborative Research grants (AJ) from The University of Melbourne are gratefully acknowledged. S.A-R. was supported by the Ministry of Agriculture and Fisheries, The Sultanate of Oman. Authors are grateful to the staff at the Laboratory of Pathology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece, for their assistance in the post mortem examinations.