Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding

Justin A Boddey; Matthew T O'Neill; Sash Lopaticki; Teresa G Carvalho; Anthony N Hodder; Thomas Nebl; Stephan Wawra; Pieter van West; Zeinab Ebrahimzadeh; Dave Richard; Sven Flemming; Tobias Spielmann; Jude Przyborski; Jeff J Babon; Alan F Cowman

Journal article

Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding

Justin A Boddey, Matthew T O'Neill, Sash Lopaticki, Teresa G Carvalho, Anthony N Hodder, Thomas Nebl, Stephan Wawra, Pieter van West, Zeinab Ebrahimzadeh, Dave Richard, Sven Flemming, Tobias Spielmann, Jude Przyborski, Jeff J Babon, Alan F Cowman

Nature Communications | NATURE PUBLISHING GROUP | Published : 2016

DOI: 10.1038/ncomms10470

University of Melbourne Researchers

Anthony Hodder Author Medical Biology (W.E.H.I.)

Alan Cowman Author Medical Biology (W.E.H.I.)

Jeffrey Babon Author Medical Biology (W.E.H.I.)

Justin Boddey Author Medical Biology (W.E.H.I.)

Grants

Awarded by National Health and Medical Research Council of Australia

Awarded by Ramaciotti Foundation Establishment Grant

Awarded by Canadian Institutes for Health Research

Awarded by German Research Foundation

Awarded by Biotechnology and Biological Sciences Research Council

Funding Acknowledgements

We thank the Red Cross blood bank in Melbourne for human erythrocytes. We thank Svenja Gunther for expression of GBP130 66-196 proteins; Michelle Gazdik and Chris Burns for help in preparing lipids; Lachlan Whitehead (Centre for Dynamic Imaging, Walter and Eliza Hall Institute) for assistance with quantification of export; and David Bocher for help with generation of STEVOR constructs. This work was supported by the National Health and Medical Research Council of Australia (grants 637406, 1010326, 1049811 and 1057960), a Ramaciotti Foundation Establishment Grant (3197/2010), a Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS, and the Canadian Institutes for Health Research (MOP#130359). J.A.B is an Australian Research Council QEII Fellow, SF was supported by the Research Training Group GRK1459 of the German Research Foundation, and AFC is a Howard Hughes International Scholar.