Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins

Esperanza Fernandez; Mark O Collins; Rachel T Uren; Maksym V Kopanitsa; Noboru H Komiyama; Mike DR Croning; Lysimachos Zografos; J Douglas Armstrong; Jyoti S Choudhary; Seth GN Grant

Journal article

Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins

Esperanza Fernandez, Mark O Collins, Rachel T Uren, Maksym V Kopanitsa, Noboru H Komiyama, Mike DR Croning, Lysimachos Zografos, J Douglas Armstrong, Jyoti S Choudhary, Seth GN Grant

Molecular Systems Biology | WILEY | Published : 2009

DOI: 10.1038/msb.2009.27

University of Melbourne Researchers

Rachel Uren Author Anatomy and Neuroscience

Grants

Funding Acknowledgements

We thank Dr M Pardo for advice on purification conditions and information on cleaved retained proteins, Dr L Yu and S Swamy for support in mass spectrometry analysis, K Porter for tissue perfusion, J Robinson and K Elsegood for mouse colony management, Dr A Enright for protein interaction data, and N Afinowi for the isolation of primary neurons. EF was supported by a Federation of European Biochemistry Societies postdoctoral fellowship; JSC, MOC, MVK, NHK, MDRC and SGNG were supported by the Wellcome Trust; RU was supported by Marie Curie Actions: Research Training Network programs. LZ was supported by the EPSRC/MRC Doctoral Training Centre in Neuroinformatics and Computational Neuroscience.