Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins
Esperanza Fernandez, Mark O Collins, Rachel T Uren, Maksym V Kopanitsa, Noboru H Komiyama, Mike DR Croning, Lysimachos Zografos, J Douglas Armstrong, Jyoti S Choudhary, Seth GN Grant
Molecular Systems Biology | WILEY | Published : 2009
We thank Dr M Pardo for advice on purification conditions and information on cleaved retained proteins, Dr L Yu and S Swamy for support in mass spectrometry analysis, K Porter for tissue perfusion, J Robinson and K Elsegood for mouse colony management, Dr A Enright for protein interaction data, and N Afinowi for the isolation of primary neurons. EF was supported by a Federation of European Biochemistry Societies postdoctoral fellowship; JSC, MOC, MVK, NHK, MDRC and SGNG were supported by the Wellcome Trust; RU was supported by Marie Curie Actions: Research Training Network programs. LZ was supported by the EPSRC/MRC Doctoral Training Centre in Neuroinformatics and Computational Neuroscience.