Journal article
Design and performance of an ultra-flexible twophoton microscope for in vivo research
JM Mayrhofer, F Haiss, D Haenni, S Weber, M Zuend, MJP Barrett, KD Ferrari, P Maechler, AS Saab, JL Stobart, MT Wyss, H Johannssen, H Osswald, LM Palmer, V Revol, CD Schuh, C Urban, A Hall, ME Larkum, E Rutz-Innerhofer Show all
Biomedical Optics Express | OPTICAL SOC AMER | Published : 2015
DOI: 10.1364/BOE.6.004228
Open access
Abstract
We present a cost-effective in vivo two-photon microscope with a highly flexible frontend for in vivo research. Our design ensures fast and reproducible access to the area of interest, including rotation of imaging plane, and maximizes space for auxiliary experimental equipment in the vicinity of the animal. Mechanical flexibility is achieved with large motorized linear stages that move the objective in the X, Y, and Z directions up to 130 mm. 360° rotation of the frontend (rotational freedom for one axis) is achieved with the combination of a motorized high precision bearing and gearing. Additionally, the modular design of the frontend, based on commercially available optomechanical parts, ..
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Funding Acknowledgements
The project was in part sponsored by the Swiss National Science Foundation and by the Hartmann Muller-Stiftung. BW and AH are members of the Clinical Research Priority Program "Molecular Imaging Network Zurich".