Journal article
Deep sequencing reveals novel Set7 networks
ST Keating, M Ziemann, J Okabe, AW Khan, A Balcerczyk, A El-Osta
Cellular and Molecular Life Sciences | SPRINGER BASEL AG | Published : 2014
Abstract
Background: Methyl-dependent regulation of transcription has expanded from a traditional focus on histones to encompass transcription factor modulation. While the Set7 lysine methyltransferase is associated with pro-inflammatory gene expression in vascular endothelial cells, genome-wide regulatory roles remain to be investigated. From initial characterization of Set7 as specific for methyl-lysine 4 of H3 histones (H3K4m1), biochemical activity toward non-histone substrates has revealed additional mechanisms of gene regulation. Results: mRNA-Seq revealed transcriptional deregulation of over 8,000 genes in an endothelial model of Set7 knockdown. Gene ontology identified up-regulated pathways i..
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Funding Acknowledgements
The authors are thankful for expert bioinformatic support by Antony Kaspi and Dr. Ross Lazarus. The authors acknowledge grant and fellowship support from the Juvenile Diabetes Research Foundation International, the Diabetes Australia Research Trust, the National Health and Medical Research Council (NHMRC) and the National Heart Foundation of Australia. STK is supported by an Australian Postgraduate Award. AE-O is a senior research fellow supported by the NHMRC. Supported in part by the Victorian Government's Operational Infrastructure Support program.