Comparative effects of GLP-1-(7-36) amide, oxyntomodulin and glucagon on rabbit gastric parietal cell function

Abstract

We have investigated in vitro, the effects of glucagon-like peptide-1-(7-36) amide), oxyntomodulin and glucagon on two rabbit parietal cell-enriched fractions (F3, F3n), with parietal cell contents of 60% and 88%, respectively. Histamine (10-5 M) stimulated [14C]aminopyrine accumulation to an amount of 850% in excess of the basal level, whereas GLP-1-(7-36) amide (10-7 M) and oxyntomodulin (10-6 M) induced increases of 50% and 30%, respectively. With a histamine concentration of 10-6 M, [14C]aminopyrine accumulation was stimulated to 498% in excess of the basal level; GLP-1-(7-36) amide (10-7 M) and oxyntomodulin (10-7 M) induced increases of 50% and 15%, respectively. With these parameters,..