Journal article
An epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator
DS Lee, JY Shin, PD Tonge, MC Puri, S Lee, H Park, WC Lee, SMI Hussein, T Bleazard, JY Yun, J Kim, M Li, N Cloonan, D Wood, JL Clancy, R Mosbergen, JH Yi, KS Yang, H Kim, H Rhee Show all
Nature Communications | Published : 2014
DOI: 10.1038/ncomms6619
Abstract
Reprogramming of somatic cells to induced pluripotent stem cells involves a dynamic rearrangement of the epigenetic landscape. To characterize this epigenomic roadmap, we have performed MethylC-seq, ChIP-seq (H3K4/K27/K36me3) and RNA-Seq on samples taken at several time points during murine secondary reprogramming as part of Project Grandiose. We find that DNA methylation gain during reprogramming occurs gradually, while loss is achieved only at the ESC-like state. Binding sites of activated factors exhibit focal demethylation during reprogramming, while ESC-like pluripotent cells are distinguished by extension of demethylation to the wider neighbourhood. We observed that genes with CpG-rich..
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Awarded by College of Medicine, Seoul National University
Funding Acknowledgements
This work has been supported by the Korean Ministry of Knowledge Economy (grant no. 10037410 to J.-S.S.), by the SNUCM research fund (grant no. 0411-20100074 to J.-S.S.) and by Macrogen Inc. (grant no. MGR03-11 and 12).