Journal article

Comprehensive transcriptome and immunophenotype analysis of renal and cardiac MSC-like populations supports strong congruence with bone marrow MSC despite maintenance of distinct identities

Rebecca A Pelekanos, Joan Li, Milena Gongora, Vashe Chandrakanthan, Janelle Scown, Norseha Suhaimi, Gary Brooke, Melinda E Christensen, Tram Doan, Alison M Rice, Geoffrey W Osborne, Sean M Grimmond, Richard P Harvey, Kerry Atkinson, Melissa H Little



Cells resembling bone marrow mesenchymal stem cells (MSC) have been isolated from many organs but their functional relationships have not been thoroughly examined. Here we compared the immunophenotype, gene expression, multipotency and immunosuppressive potential of MSC-like colony-forming cells from adult murine bone marrow (bmMSC), kidney (kCFU-F) and heart (cCFU-F), cultured under uniform conditions. All populations showed classic MSC morphology and in vitro mesodermal multipotency. Of the two solid organ-specific CFU-F, only kCFU-F displayed suppression of T-cell alloreactivity in vitro, albeit to a lesser extent than bmMSC. Quantitative immunophenotyping using 81 phycoerythrin-conjugate..

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Funding Acknowledgements

This work was supported by the Australian Stem Cell Centre (grants to K.A., M.L, R.H. and S.G.), the Mater Medical Research Institute/Mater Foundation (KA) and NHMRC (AMR). The microarray research was supported by the Australian Research Council Special Research Centre for Functional and Applied Genomics (Institute for Molecular Bioscience) Microarray Facility. Technical assistance in immunophenotyping was provided by John Wilson and Virginia Nink from the Queensland Brain Institute Flow Cytometry Facility, University of Queensland. Further technical assistance was supplied by Robert Wadley. AMR is a Queensland Government Smart Futures Fellow. ML and SG are Research Fellows and RH is an Australia Fellow with the National Health and Medical Research Council, Australia.