Use of DNA-Damaging Agents and RNA Pooling to Assess Expression Profiles Associated with BRCA1 and BRCA2 Mutation Status in Familial Breast Cancer Patients
Logan C Walker, Bryony A Thompson, Nic Waddell, Sean M Grimmond, Amanda B Spurdle
PLOS GENETICS | PUBLIC LIBRARY SCIENCE | Published : 2010
A large number of rare sequence variants of unknown clinical significance have been identified in the breast cancer susceptibility genes, BRCA1 and BRCA2. Laboratory-based methods that can distinguish between carriers of pathogenic mutations and non-carriers are likely to have utility for the classification of these sequence variants. To identify predictors of pathogenic mutation status in familial breast cancer patients, we explored the use of gene expression arrays to assess the effect of two DNA-damaging agents (irradiation and mitomycin C) on cellular response in relation to BRCA1 and BRCA2 mutation status. A range of regimes was used to treat 27 lymphoblastoid cell-lines (LCLs) derived ..View full abstract
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This work was supported by the National Health and Medical Research Council of Australia (NHMRC). LCW, NW, and BAT were supported by grant funding from the NHMRC. ABS and SMG are NHMRC Senior Research Fellows. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.