Regulated post-transcriptional RNA cleavage diversifies the eukaryotic transcriptome
Tim R Mercer, Marcel E Dinger, Cameron P Bracken, Gabriel Kolle, Jan M Szubert, Darren J Korbie, Marjan E Askarian-Amiri, Brooke B Gardiner, Gregory J Goodall, Sean M Grimmond, John S Mattick
GENOME RESEARCH | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2010
The complexity of the eukaryotic transcriptome is generated by the interplay of transcription initiation, termination, alternative splicing, and other forms of post-transcriptional modification. It was recently shown that RNA transcripts may also undergo cleavage and secondary 5' capping. Here, we show that post-transcriptional cleavage of RNA contributes to the diversification of the transcriptome by generating a range of small RNAs and long coding and noncoding RNAs. Using genome-wide histone modification and RNA polymerase II occupancy data, we confirm that the vast majority of intraexonic CAGE tags are derived from post-transcriptional processing. By comparing exonic CAGE tags to tissue-..View full abstract
Awarded by Australian Research Council/University of Queensland
Awarded by National Health and Medical Research Council of Australia
This work was supported by grants and fellowships from the Australian Research Council/University of Queensland cosponsored Federation Fellowship (FF0561986; J.S.M.), a National Health and Medical Research Council of Australia Career Development Award (CDA631542; M.E.D.), a Queensland Government Department of Employment, Economic Development and Innovation Smart Futures Fellowship (M.E.D.), and the Australian Stem Cell Centre.