Journal article
MicroRNAs and their isomiRs function cooperatively to target common biological pathways
N Cloonan, S Wani, Q Xu, J Gu, K Lea, S Heater, C Barbacioru, AL Steptoe, HC Martin, E Nourbakhsh, K Krishnan, B Gardiner, X Wang, K Nones, JA Steen, NA Matigian, DL Wood, KS Kassahn, N Waddell, J Shepherd Show all
Genome Biology | Published : 2011
Abstract
Background: Variants of microRNAs (miRNAs), called isomiRs, are commonly reported in deep-sequencing studies; however, the functional significance of these variants remains controversial. Observational studies show that isomiR patterns are non-random, hinting that these molecules could be regulated and therefore functional, although no conclusive biological role has been demonstrated for these molecules.Results: To assess the biological relevance of isomiRs, we have performed ultra-deep miRNA-seq on ten adult human tissues, and created an analysis pipeline called miRNA-MATE to align, annotate, and analyze miRNAs and their isomiRs. We find that isomiRs share sequence and expression characteri..
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Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by Australian Research Council (ARC) Discovery Project Grants DP0988754 and DP1093164. NC is supported by an ARC Postdoctoral Fellowship, and SMG is supported by an Australian National Health and Medical Research Council (NHMRC) Senior Research Fellowship. We are grateful for the helpful discussions with all members of QCMG, and we are particularly thankful to JV Pearson, DF Taylor, and S Wood for HPC infrastructure and support. We also thank Ryan Taft, Selene Fernandez Valverde, and John Mattick for helpful discussions and advice.