Journal article
Reduced mucin sulfonation and impaired intestinal barrier function in the hyposulfataemic NaS1 null mouse
PA Dawson, S Huxley, B Gardiner, T Tran, JL McAuley, S Grimmond, MA McGuckin, D Markovich
Gut | BMJ PUBLISHING GROUP | Published : 2009
Abstract
Objective: Sulfate (SO42-) is an abundant component of intestinal mucins and its content is decreased in certain gastrointestinal diseases, including inflammatory bowel disease. In this study, the hyposulfataemic NaS1 sulfate transporter null (Nas1-/-) mice were used to investigate the physiological consequences of disturbed sulfate homeostasis on (1) intestinal sulfomucin content and mRNA expression; (2) intestinal permeability and proliferation; (3) dextran sulfate sodium (DSS)-induced colitis; and (4) intestinal barrier function against the bacterial pathogen, Campylobacter jejuni. Methods: Intestinal sulfomucins and sialomucins were detected by high iron diamine staining, permeability wa..
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Funding Acknowledgements
We thank Drs T Walker and H Cooper for advice on qRT- PCR. SG is a recipient of an NHMRC career development award. MM is a recipient of a Senior Research Fellowship from The Cancer Council of Queensland. The array reagents were provided by the Australian Cancer Research Foundation DNA microarray initiative. This work was supported in part by the Australian Research Council and the National Health and Medical Research Council.