Journal article

Redirection of renal mesenchyme to stromal and chondrocytic fates in the presence of TGF-beta 2

Sunder Sims-Lucas, Richard J Young, Gemma Martinez, Darrin Taylor, Sean M Grimmond, Rohan Teasdale, Melissa H Little, John F Bertram, Georgina Caruana



Many members of the transforming growth factor-beta (TGF-beta) superfamily have been shown to be important regulators of metanephric development. In this study, we characterized the effect of TGF-beta2 on metanephric development. Rat and mouse metanephroi cultured in the presence of exogenous TGF-beta2 for up to 15 days were small, and contained rudimentary ureteric branches and few glomeruli. These metanephroi were mostly comprised of mesenchymal cells, with two cell populations (designated Type 1 and Type 2 cells) evident. Type 1 cells were only observed when TGF-beta2 was added from the commencement of culture, they resembled chondroblasts and were Alcian Blue and Col IIB positive. Type 2..

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