Redirection of renal mesenchyme to stromal and chondrocytic fates in the presence of TGF-beta 2
Sunder Sims-Lucas, Richard J Young, Gemma Martinez, Darrin Taylor, Sean M Grimmond, Rohan Teasdale, Melissa H Little, John F Bertram, Georgina Caruana
DIFFERENTIATION | ELSEVIER SCI LTD | Published : 2010
Many members of the transforming growth factor-beta (TGF-beta) superfamily have been shown to be important regulators of metanephric development. In this study, we characterized the effect of TGF-beta2 on metanephric development. Rat and mouse metanephroi cultured in the presence of exogenous TGF-beta2 for up to 15 days were small, and contained rudimentary ureteric branches and few glomeruli. These metanephroi were mostly comprised of mesenchymal cells, with two cell populations (designated Type 1 and Type 2 cells) evident. Type 1 cells were only observed when TGF-beta2 was added from the commencement of culture, they resembled chondroblasts and were Alcian Blue and Col IIB positive. Type 2..View full abstract
The authors would like to thank Johnson and Johnson Research for their support and Ian Boundy for his histology expertise. SS-L would like to thank Peter Lucas for all his efforts. ML is an NHMRC Principal Research Fellow.