Journal article

Experimental validation of the regulated expression of large numbers of non-coding RNAs from the mouse genome

T Ravasi, H Suzuki, KC Pang, S Katayama, M Furuno, R Okunishi, S Fukuda, KL Ru, MC Frith, MM Gongora, SM Grimmond, DA Hume, Y Hayashizaki, JS Mattick

Genome Research | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2006

Abstract

Recent large-scale analyses of mainly full-length cDNA libraries generated from a variety of mouse tissues indicated that almost half of all representative cloned sequences did not contain an apparent protein-coding sequence, and were putatively derived from non-protein-coding RNA (ncRNA) genes. However, many of these clones were singletons and the majority were unspliced, raising the possibility that they may be derived from genomic DNA or unprocessed pre-mRNA contamination during library construction, or alternatively represent nonspecific "transcriptional noise." Here we show, using reverse transcriptase-dependent PCR, microarray, and Northern blot analyses, that many of these clones were..

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University of Melbourne Researchers