Journal article
Kidney transcriptome reveals altered steroid homeostasis in NaS1 sulfate transporter null mice
PA Dawson, B Gardiner, S Lee, S Grimmond, D Markovich
Journal of Steroid Biochemistry and Molecular Biology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2008
Abstract
Sulfate is essential for human growth and development, and circulating sulfate levels are maintained by the NaS1 sulfate transporter which is expressed in the kidney. Previously, we generated a NaS1-null (Nas1-/-) mouse which exhibits hyposulfatemia. In this study, we investigated the kidney transcriptome of Nas1-/- mice. We found increased (n = 25) and decreased (n = 60) mRNA levels of genes with functional roles that include sulfate transport and steroid metabolism. Corticosteroid-binding globulin was the most up-regulated gene (110% increase) in Nas1-/- mouse kidney, whereas the sulfate anion transporter-1 (Sat1) was among the most down-regulated genes (≥50% decrease). These findings led ..
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Funding Acknowledgements
We thank Drs. M. Waters, T. Walker and H. Cooper (University of Queensland, Brisbane, Australia) for valuable discussions. Cbg and Cyp11a1 antibodies were kind gifts from Drs. G.L. Hammond (Child and Family Research Institute, Vancouver, Canada) and B. Chung (Institute of Molecular Biology, Academia Sinica, Nankang, Taiwan), respectively. This work was supported by the Australian National Health and Medical Research Council, Australian Research Council, and a Project grant awarded by the Queensland Cancer Fund. S. Lee is a recipient of the University of Queensland Confirmation Scholarship. S. Grimmond is a recipient of an NHMRC career development award. The array reagents were provided by the Australian Cancer Research Foundation DNA microarray initiative.