Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene

I Lemmens; J Merregaert; WJM Van De Ven; K Koen; CX Zhang; S Giraud; V Wautot; N Buisson; K De Witte; J Salandre; G Lenoir; A Calender; F Parente; D Quincey; A Courseaux; GF Carle; P Gaudray; MJ De Wit; CJM Lips; JWM Höppener; S Khodaei; AL Grant; G Weber; S Kytölä; BT Teh; F Farnebo; S Grimmond; C Phelan; C Larsson; SA Forbes; JHD Bassett; AAJ Pannett; RV Thakker

Journal article

Construction of a 1.2-Mb sequence-ready contig of chromosome 11q13 encompassing the multiple endocrine neoplasia type 1 (MEN1) gene

I Lemmens, J Merregaert, WJM Van De Ven, K Koen, CX Zhang, S Giraud, V Wautot, N Buisson, K De Witte, J Salandre, G Lenoir, A Calender, F Parente, D Quincey, A Courseaux, GF Carle, P Gaudray, MJ De Wit, CJM Lips, JWM Höppener Show all

Genomics | Published : 1997

DOI: 10.1006/geno.1997.4872

Abstract

Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant familial cancer syndrome characterized by parathyroid, pancreatic, and anterior pituitary tumors. The MEN1 locus has been previously localized to chromosome 11q13, and a 2-Mb gene-rich region flanked by D11S1883 and D11S449 has been defined. We have pursued studies to facilitate identification of the MEN1 gene by narrowing this critical region to a 900-kb interval between the VRF and D11S1783 loci through meiotic mapping. This was achieved by investigating 17 cosmids for microsatellite polymorphisms, which defined two novel polymorphisms at the VRF and A0138 loci, and utilizing these to characterize recombinants in MEN1 fami..

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