Journal article
A Genomic Approach to Resolving Relapse versus Reinfection among Four Cases of Buruli Ulcer
M Eddyani, K Vandelannoote, CJ Meehan, S Bhuju, JL Porter, J Aguiar, T Seemann, M Jarek, M Singh, F Portaels, TP Stinear, BC de Jong
Plos Neglected Tropical Diseases | Published : 2015
Abstract
Background: Increased availability of Next Generation Sequencing (NGS) techniques allows, for the first time, to distinguish relapses from reinfections in patients with multiple Buruli ulcer (BU) episodes. Methodology: We compared the number and location of single nucleotide polymorphisms (SNPs) identified by genomic screening between four pairs of Mycobacterium ulcerans isolates collected at the time of first diagnosis and at recurrence, derived from a collection of almost 5000 well characterized clinical samples from one BU treatment center in Benin. Principal Findings: The findings suggest that after surgical treatment—without antibiotics—the second episodes were due to relapse rather tha..
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Funding Acknowledgements
This work was supported by the UBS Optimus Foundation (Zurich, Switzerland) and the Department of Economy, Science and Innovation of the Flemish Government (Belgium). KV was supported by a VLADOC PhD scholarship of VLIR-UOS (Belgium). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.