Intradermal delivery of DNA encoding HCV NS3 and perforin elicits robust cell-mediated immunity in mice and pigs
B Grubor-Bauk, W Yu, D Wijesundara, J Gummow, T Garrod, AJ Brennan, I Voskoboinik, EJ Gowans
Gene Therapy | NATURE PUBLISHING GROUP | Published : 2016
Awarded by National Health and Medical Research Council (NHMRC) of Australia
Awarded by Australian-Indian biotechnology fund
We thank Dr Paul Radspinner (FluGen Inc., Madison, WI, USA) who provided the microneedle device and Renee Herber for training to use the device. We also thank Dr John Taylor (University of Auckland, New Zealand) and Dr Barbara Coulson (University of Melbourne, Australia) for the gift of the NSP4 construct. We acknowledge the kind gift of the HCV peptide pools, which were obtained through the AIDS Reagent and Reference Reagent Program, Division of AIDS, NIAID, National Institutes of Health, USA. We also thank Dr Stuart Howell for statistical advice. This research was supported by grants APP1026293, 543139 and 543143 from the National Health and Medical Research Council (NHMRC) of Australia, grant BF040005 from the Australian-Indian biotechnology fund and a grant from the Hospital Research Foundation (THRF). DW is a Research Fellow supported by THRF.