Journal article
Transcriptional analysis of T cells resident in human skin
J Li, M Olshansky, FR Carbone, JZ Ma
Plos One | Published : 2016
Abstract
Human skin contains various populations of memory T cells in permanent residence and in transit. Arguably, the best characterized of the skin subsets are the CD8+ permanently resident memory T cells (TRM) expressing the integrin subunit, CD103. In order to investigate the remaining skin T cells, we isolated skin-tropic (CLA+) helper T cells, regulatory T cells, and CD8+ CD103- T cells from skin and blood for RNA microarray analysis to compare the transcriptional profiles of these groups.We found that despite their common tropism, the T cells isolated from skin were transcriptionally distinct from blood-derived CLA+ T cells. A shared pool of genes contributed to the skin/blood discrepancy, wi..
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Funding Acknowledgements
[ "The work was supported by grants from the Australian National Health and Medical Research Council to FRC. JL is a recipient of the Elizabeth and Vernon Puzey Scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.", "We thank all patients for participating in this study, and the team at the Royal Women's Hospital for assistance in obtaining tissue samples. We are grateful to Dr. D Fernandez-Ruiz for help in compiling the T<INF>RM</INF> gene set lists. Biospecimens and data used in this research were obtained from the Victorian Cancer Biobank, Victoria, Australia, with appropriate ethics approval. The Victorian Cancer Biobank is supported by the Victorian Government. We thank Dr. K Shaw for assistance with proofreading." ]