Journal article
A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth
JHA Vissers, SA Manning, A Kulkarni, KF Harvey
Nature Communications | Published : 2016
DOI: 10.1038/ncomms10368
Abstract
Libraries of transgenic Drosophila melanogaster carrying RNA interference (RNAi) constructs have been used extensively to perform large-scale functional genetic screens in vivo. For example, RNAi screens have facilitated the discovery of multiple components of the Hippo pathway, an evolutionarily conserved growth-regulatory network. Here we investigate an important technical limitation with the widely used VDRC KK RNAi collection. We find that approximately 25% of VDRC KK RNAi lines cause false-positive enhancement of the Hippo pathway, owing to ectopic expression of the Tiptop transcription factor. Of relevance to the broader Drosophila community, ectopic tiptop (tio) expression can also ca..
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Funding Acknowledgements
We thank Edward Green for helpful comments, Nan Hu and Barry Denholm for UAS-tio flies and members of the Harvey laboratory and Kate Sutherland for discussions and critical reading of the manuscript. We thank the Vienna Drosophila RNAi Center, the Bloomington Drosophila Stock Centre, the National Institute of Genetics, Japan, the Australian Drosophila Research Support Facility (www.ozdros.com), and the Developmental Studies Hybridoma Bank for fly stocks and antibodies. K.F.H is a National Health and Medical Research Council Senior Research Fellow. S.A.M. was supported by a 2012 Australian Postgraduate Award scholarship. A.K. was supported by a Cancer Council Victoria Vacation Studentship. This research was supported by a Project Grant from the National Health and Medical Research Council of Australia.