Journal article
Genomic characterisation of small cell lung cancer patient-derived xenografts generated from endobronchial ultrasound-guided transbronchial needle aspiration specimens
TL Leong, KD Marini, FJ Rossello, SN Jayasekara, PA Russell, Z Prodanovic, B Kumar, V Ganju, M Alamgeer, LB Irving, DP Steinfort, CD Peacock, JE Cain, A Szczepny, D Neil Watkins
Plos One | Published : 2014
Abstract
Patient-derived xenograft (PDX) models generated from surgical specimens are gaining popularity as preclinical models of cancer. However, establishment of PDX lines from small cell lung cancer (SCLC) patients is difficult due to very limited amount of available biopsy material. We asked whether SCLC cells obtained from endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) could generate PDX lines that maintained the phenotypic and genetic characteristics of the primary tumor. Following successful EBUS-TBNA sampling for diagnostic purposes, we obtained an extra sample for cytologic analysis and implantation into the flanks of immunodeficient mice. Animals were monitored..
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Funding Acknowledgements
This work was supported by the Victorian Cancer Agency (www.victoriancanceragency.org.au), the National Health and Medical Research Council of Australia (www.nhmrc.gov.au), and the Victorian Government Operational Infrastructure Support Program (www.business.vic.gov.au/grants-and-assistance/programs/medical-research-operational-infrastructure-program). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.