Recombinant influenza virus expressing HIV-1 p24 capsid protein induces mucosal HIV-specific CD8 T-cell responses
Hyon-Xhi Tan, Brad P Gilbertson, Sinthujan Jegaskanda, Sheilajen Alcantara, Thakshila Amarasena, John Stambas, Julie L McAuley, Stephen J Kent, Robert De Rose
VACCINE | ELSEVIER SCI LTD | Published : 2016
Influenza viruses are promising mucosal vaccine vectors for HIV but their use has been limited by difficulties in engineering the expression of large amounts of foreign protein. We developed recombinant influenza viruses incorporating the HIV-1 p24 gag capsid into the NS-segment of PR8 (H1N1) and X31 (H3N2) influenza viruses with the use of multiple 2A ribosomal skip sequences. Despite the insertion of a sizable HIV-1 gene into the influenza genome, recombinant viruses were readily rescued to high titers. Intracellular expression of p24 capsid was confirmed by in vitro infection assays. The recombinant influenza viruses were subsequently tested as mucosal vaccines in BALB/c mice. Recombinant..View full abstract
This research was supported by the Australian Research Council and National Health and Medical Research Council, Australia. We thank St. Jude Children's Hospital, Memphis, TN, for providing the pHW2000 plasmid. We also thank Wendy Winnall for her insightful advice for the statistical analysis of this study.